Process for the production of a standard for the detection of protein aggregates of a protein misfolding disease and standard and its use

摘要

Method for the production of a standard for the detection of protein aggregates of a protein misfolding disease, with the steps: A) providing an inorganic nanoparticle with the size of the protein aggregate of the protein misfolding disease, B) formation of free amino groups on the surface of the nanoparticle, for the functionalization of the nanoparticle surface to one amine-functionalized nanoparticles, C) i) binding of maleimido spacer carboxylic acid to the free amino groups in step B), or ii) binding of maleimido spacer carboxylic acid, which has been converted into an NHS ester, to the free amino group of step B) , D) Binding of monomers of the protein aggregate i) to the maleimido spacer carboxylic acids via a sulfhydryl group at the free end of the monomers, or ii) to the NHS ester via the amino group at the free end of the monomer.