METHOD FOR DETERMINING THE FUNCTIONAL ACTIVITY OF THE HUMAN COMPLEMENT SYSTEM FOR PREDICTING THE SEVERITY OF THE COURSE OF A SYSTEMIC INFLAMMATORY REACTION

摘要

FIELD: diagnostics.;SUBSTANCE: invention relates to diagnostics, namely to a method for determining the functional activity of the classical pathway of the human complement system for predicting the severity of the course of the systemic inflammatory response. A method for determining the functional activity of the classical pathway of the human complement system for predicting the severity of the course of a systemic inflammatory reaction includes carrying out a lysis reaction of human erythrocytes of group A of the AB0 system (E(A)), sensitized with anti-A monoclonal IgM antibodies (E(A)mAb) and serum under conditions of veronal saline buffer (VBS2+), incubation of the obtained sample, then the reaction of complement-dependent lysis of E(A)mAb is evaluated turbidimetrically, by reducing the optical density of the suspension E(A)mAb at a wavelength of 620 nm determines the degree of erythrocyte lysis in experimental samples according to the calibration graph, where the control E(A)mAb represents 0% lysis, and the control of complete lysis E(A)mAb is 100% lysis, under certain conditions, while the increased functional activity of the classical pathway of the human complement system is noted with a degree of lysis of more than 60%, with a degree lysis from 31% to 59% as normal and with a degree of lysis less than 30% as reduced functional activity of the human complement system.;EFFECT: above-described method allows increasing the accuracy of the method for studying the functional activity of the classical pathway of the complement system, as well as to simplify the registration of the results of the analysis of the functional activity of the classical pathway of the complement system in routine studies and adaptation of the method for screening studies in the conditions of clinical diagnostic laboratories.;1 cl, 5 dwg, 2 tbl, 2 ex