UV quantitative label-free DNA amplification detection

摘要

The present invention amplifies nucleic acid molecules by polymerase chain reaction (PCR) or loop-mediated isothermal amplification (LAMP), through bulk heating of a biological enzymatic reaction mixture in a solution containing a nucleic acid template, polymerase, and chemically modified nanoparticles. and to a method and system for quantifying its amplification. The methods and systems may include quantifying the amplification by irradiating the biological enzyme reaction mixture with an ultraviolet (UV) light source during the annealing and/or elongation step.