Linear multifunctional multimer biomolecule bound to polyubiquitin linker and its uses

摘要

The present invention obtains a biomolecule to which a ubiquitin C-terminal tag is bound by recombinantly expressing it from a host cell, and obtains this from an E1 (Activation enzyme), E2 (Conjugation enzyme), or E3 (Ligase) protein involved in ubiquitination. To provide a linear multimer biomolecule polymer formed by binding a biomolecule to a polyubiquitin scaffold in which two or more ubiquitins are covalently formed by polyubiquitinization in vitro with a substrate and a substrate. do. The polymer according to the present invention can be used for separation / purification of biomolecules, separation of target substances that bind to the biomolecules, and the like. [Selection diagram] None