PCR primer set for environmental DNA detection of Culter brevicauda and method using the same

摘要

The present invention relates to a method for discriminating swanfish from other freshwater fish by amplifying a target sequence specific for swanfish using a primer set consisting of the nucleotide sequence shown in SEQ ID NO:3 and SEQ ID NO:4, which are PCR primers. In addition, excellent specificity of swanfish from environmental water through real-time polymerase chain reaction (RT-PCR) including a probe consisting of SEQ ID NO: 3 and 4 primer sets and the nucleotide sequence shown in SEQ ID NO: 5 It relates to a method for rapid detection with high sensitivity.