Formation of planar intestinal crypt arrays with stem cells / proliferative cell compartments and differentiated cell zones

摘要

A method of generating tissue components comprising two or more distinct regions including different cell populations or cell lines is described.The method is to provide a support substrate or substrate assembly that includes two or more physically distinct regions, wherein two or more physically distinct regions of a support substrate or substrate assembly are different from each other, and the steps described above The method of depositing and disposing one or more cells on a support substrate or substrate assembly.The cells can have at least two zones (e.g., proliferating zones and non proliferation zones) to form a continuous monolayer operable as an in vitro intestinal model.This model is two-dimensional, thus facilitating quick and easy imaging.Methods for measuring the effects of drugs, dietary supplements, and metabolites on intestinal cells using systems including tissue components and tissue components are also described.