GENE EXPRESSION COMPONENT, CLONING VECTOR CONSTRUCTED THEREBY AND APPLICATION

摘要

A gene expression component, a cloning vector constructed thereby and an application. The gene expression component encodes β-galactosidase α peptide and ω peptide; the gene expression component comprises a constitutive promoter, and the constitutive promoter promotes expressed LacZα gene and LacZω gene. A constructed pWizard plasmid can produce substances having β-galactosidase activity in a host cell without IPTG induction, the application scope of the host cell is expanded, the pWizard plasmid is a high-copy plasmid, and therefore, the copy number of the LacZα gene and the LacZω gene in a host bacterium is high, the expression level of the host cell to an enzyme having β-galactosidase activity is significantly increased, the color development effect and the color development speed are enhanced, and the color development time is shortened.