A FAST AND SCALABLE MAMMALIAN MOTOR NEURON DIFFERENTIATION SYSTEM FOR MODELING NEUROPATHY

摘要

Described herein are suspension culture-based, directed differentiation protocols, methods, and systems useful in producing a highly pure MN population within 3 weeks. Also disclosed are compositions produced by the disclosed methods, protocols, processes, and systems, and compositions useful in their implementation. The resulting MN populations express surface markers that may be consistent with a motor neuron phenotype. In many embodiments, the phenotype may be specific for motor neurons from the thoracic and lumbar intersection of a mammal. Also disclosed is an effective model system for the investigation of RNA transcript localization in MN soma and neurites in an unbiased high-throughput manner.