Continuous culture of Aspergillus niger and method for producing citric acid using the same

摘要

An object of the present invention is to provide a continuous culture of black aspergillus inoculum and a method for producing citric acid using the same. The present invention discloses a method for continuously culturing a black aspergillus inoculum, wherein the method is (1) a start step: a step of inoculating a spore of the black aspergillus inoculum into an inoculum to obtain a liquid inoculum, and (2) a continuous culture step of the inoculum. : A step of continuously dispersing the liquid inoculum obtained in step (1), continuously culturing the liquid inoculum obtained by dispersion, and at the same time replenishing a fresh inoculum inoculum, and (3) a stop step: fresh. It includes a step of stopping the replenishment and dispersion treatment of the inoculum inoculum, continuing the inoculation to obtain a liquid inoculum, and then transferring the inoculum to the inoculum for fermentation. The method of the present invention solves the problem of slow growth of multicellular filamentous fungi and easy loss during continuous culture of mycelium, completely realizes continuous culture of inoculum, and optimizes growth environment for mycelia. It keeps the inoculum inside, prevents the inoculation of the inoculum, keeps the liquid inoculum in a continuously stable and vibrant state, and improves the citric acid production performance by the corresponding fermentation.