Method and application for screening target specific nuclease using multitarget system of on target and off target

摘要

The present invention relates to methods for selecting target-specific nucleases with high specificity and high activity, and more particularly, using a multi-target system capable of simultaneously identifying off-target activity and on-target activity. Methods for screening and selecting target specific nuclease systems. According to the present invention, target specific nucleases can be selected which have a reduced off target effect and an increased on target effect.