Method For Screening Target Specific Nuclease Using Multi-Target System Of On-Target And Off-Target And Use Thereof

摘要

The present invention relates to a method for selecting a target specific nuclease having high specificity and high activity and, more particularly, to a method for screening and selecting a target specific nuclease system by using a multiple target system capable of simultaneously identifying off-target activity and on-target activity. Through the present invention, it is possible to select a target specific nuclease which has a decreased off-target effect and an increased on-target effect.