METHOD FOR CULTURING PUERARIA THOMSONII BY PROLIFERATION OF SINGLE-BUD STEM SEGMENT AND ONE-STEP SEEDLING FORMATION

摘要

Disclosed is a method for culturing Pueraria thomsonii by proliferation of a single-bud stem segment and one-step seedling formation. The method specifically comprises the following steps: S1, explant treatment: selecting a tender vine that grows well and has no diseases or insect pests, immersing and disinfecting same, and then washing the vine with sterile water; finally, absorbing moisture on the surface of a stem segment for later use by means of using sterile filter paper; S2, culture of a sterile seedling: under sterile conditions, cutting the vine treated in step S1 into a stem segment with a bud; vertically inserting a biological lower end into a primary culture medium for culture, so as to obtain the sterile seedling; S3: proliferation and rooting induction of the single-bud stem segment: under sterile conditions, cutting the sterile seedling obtained in step S2 to obtain a single-bud stem segment with an axillary bud, and transferring the single-bud stem segment into an intermittent submerged culture device for multiplication and rooting culture; S4, transplanting and survival of a tissue culture seedling: transferring a sterile bottle seedling that is 3-4 cm high, grows well, and has well developed roots from a culture room to a greenhouse for seedling hardening; and then, taking out the seedling, washing away a liquid culture medium attached to the seedling, and planting the seedling in a nutrition pot.