MULTIPLEXED COLOCALIZATION-BY-LINKAGE ASSAYS FOR THE DETECTION AND ANALYSIS OF ANALYTES

摘要

Provided herein are colocalization-by-linkage assay (CLA) compositions and methods for multiplexed analysis of an analyte or analytes. The CLA compositions and methods, as described herein, are engineered to detect multiple analytes using multiple detection reagents coupled to a single support. Readout or detection sensitivity is achieved through the use of release-dependent transduction (RDT) or displacer-dependent transduction. Further multiplexing can also be achieved through the use of barcoded elements used in the capture and detection of an analytes.