De novo design of protein switches for tunable control of protein degradation

摘要

Disclosed herein are non-naturally occurring cage polypeptides, kits and degron LOCKRs, including cage polypeptides, and uses thereof, wherein the cage polypeptide comprises (a) a helix comprising 2 to 7 alpha-helices. A bundle comprising: (i) a structural region; and (ii) a latch region, wherein the latch region comprises a degron positioned within the latch region, wherein the structural region interacts with the latch region to prevent activation of the degron; and (b) an amino acid linker connecting each alpha helix.