PRIME EDITING TECHNOLOGY FOR PLANT GENOME ENGINEERING

摘要

The invention relates to a method for inserting a desired edit at a target site in a double-stranded DNA sequence in a plant cell, using a Cas nickase associated with a reverse transcriptase adapted to plants and a prime-editing guide RNA comprising in particular a guide RNA region to provide targeting, a template RNA containing the desired edit and a primer binding site (PBS) for the reverse transcriptase.