ZMPHYCS MUTANT PROTEIN RELATED TO FLOWERING PERIOD OF MAIZE, ENCODING GENE, RECOMBINANT VECTOR AND USE THEREOF

摘要

A ZmPHYCs mutant protein related to a flowering period of maize, an encoding gene, a recombinant vector, and use thereof. A CRISPR/Cas9 gene editing technology is used for mutating maize ZmPHYC1 and ZmPHYC2 genes together to create a novel early-flowering maize allele and delete an exogenous sequence of a CRISPR vector so as to obtain a strain with phenotype-stable inheritance. A functional deletion double mutant thereof exhibits an early-flowering phenotype under long-day conditions, so that it is determined that the ZmPHCYs mutant protein produced after the simultaneous mutation of the maize ZmPHYC1 and ZmPHYC2 genes have the effect of promoting the flowering of maize under long-day conditions. The present invention has great significance for regulating the flowering period of maize, can also be applied to maize inbred line improvement and cross breeding, and has advantages such as precision and high efficiency compared with conventional breeding.