SINGLE PASS TANGENTIAL FLOW FILTRATION HYBRID CONFIGURATIONS FOR ENHANCING CONCENTRATION OF MACROMOLECULE SOLUTIONS

摘要

This disclosure provides a method for concentrating a solution of a macromolecule that is retained on at least two semi-permeable membranes that have different molecular weight cutoffs (MWCOs), the method comprising passing the solution through a hybrid configuration of said semi-permeable membranes staged in series in a single pass tangential flow filtration (SPTFF) apparatus. The method is applicable to the efficient concentration of biological macromolecules such as proteins, antibodies and nucleic acids.