DYNAMIC MULTIPHASE REACTION IN ONE-POT FOR CRISPR/CAS-DERIVED ULTRA-SENSITIVE MOLECULAR DETECTION

摘要

Described herein is an aqueous, miscible, multiphase, one-pot detection system including a first phase comprising a low density solution comprising a nucleic acid detection system; and a second phase in diffusive communication with the first phase, the second phase having a higher density than the first phase, and the second phase including a nucleic acid amplification system. Also included are multiwell plates and/or devices including the system and methods of detecting target nucleic acids.