Problem to be solved: including a sample module, a microfluidic nucleic acid amplification device, and an analysis apparatus Problem to be solved: to provide a system for facilitating a fully automated Nestler combiner polymerase amplification (RPA) in a sample delivered to a nucleic acid amplification device via a sample module.First reaction chamber for performing first nucleic acid amplificationAndIncludes one or more second reaction chambers to perform second nucleic acid amplificationCard bodyA passage for supplying the sample fluid to the first reaction chamberOne or more detection chambers connected to the one or more second reaction chambersAndIncluding a detection module coupled to each detection chamberDiagnosis card.Fig.19
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