APPARATUS AND PROCESS FOR THE AUTOMATED MANUFACTURING OF GENETICALLY ENGINEERED CELLS FROM BIOLOGICAL FLUIDS

摘要

An automated apparatus suitable for the preparation of genetically transformed cells from biological fluids including of an operating unit and a rotating concentric hollow processing chamber able to automatically process biological samples, such as white blood cells from apheresis, bone marrow or cultured cells, to manufacture genetically engineered cells. The operating unit is rotatable around its center about a horizontal axis and the processing chamber is rotatable about its central rotor axis perpendicular with the rotating axis of the operating unit. The processing chamber can be operated at high rotating speeds when oriented vertically to generate centrifugal force on the contained sample allowing separation of solids from liquids and at low speeds or by series of fast sequential clockwise and counterclockwise rotations when oriented horizontally for incubation, solid resuspension and magnetic beads magnetic attachment.