Humanized rodents for testing drugs

摘要

It is a method and composition of an in vivo test of a drug containing human FC in a genetically modified rodent (e.g., testing of pharmacokinetics and / or pharmacodynamic properties of such therapeutic agents in genetically modified rodents).In some embodiments, genetically modified rodents express antibodies including human Fc (e.g., human IgG1 FC, human igg4fc).In some embodiments, the rodent has a complete human antibody γ Or κ） Is expressed.In certain embodimentsGenetically modified rodentsOne or more Fc receptors containing human extracellular domainsFetal Fc receptor (FcRn),β－ 2 - microglobulin polypeptide β 2m)FC ε Receptor 1 α（ FC ε R1 α）、FC γ Receptor 1 alpha γ R1a,Fc gamma receptor 2A γ R2A)Fc gamma receptor 2B γ R2b,Fc gamma receptor 3A (FC) γ R3a,Fc gamma receptor 3B γ R3b,Fc gamma receptor 2C (FC) γ Contains r2c.The transmembrane domain and cytoplasmic domain of such receptors may be human or non human beings (e.g. rodents).Diagram