TARGETED DRUG DELIVERY SYSTEM USING BACTERIAL GHOSTS OF SALMONELLA TOWARD HEPATIC CANCER CELLS

摘要

Bacterial ghosts are biological vehicles of dead and evacuated bacteria from their internal components which preserved the external characters. Recently, bacterial ghosts are considered as a biotechnology platform for some biomedical applications such as drug delivery systems and vaccination. In this invention, the loaded bacterial ghosts of Salmonella typhimurium with doxorubicin (DOX) were used as a targeted drug delivery system (dosage form) which loaded by application of specific conditions. The DOX loaded bacterial ghosts were targeted toward human hepatic cancer cell line (HepG2) in-vitro and achieved antiproliferative activity less than the free DOX by three folds, which suggests less DOX side effects. Additionally, the DOX loaded bacterial ghosts of Salmonella achieved about 91% apoptosis in hepatic cancer cells while the free DOX achieved about 39% apoptosis all in concentration of (1 µg/ml).