Problem to be solved: to provide a pressurizing apparatus to be applied to a particle separating apparatus capable of conveniently reducing the burden of operation and realizing a particle separation device capable of appropriately separating particles.The compressed air discharged from the air compressing unit 110 passes through a compressed air flow tube 120 and a branch pipe 121, and is sent to a sample liquid receiving portion 4 containing a sample liquid and a buffer liquid accommodating portion 5 accommodating a buffer solution. The sample liquid and buffer liquid are introduced into the DLD microchannel chip 60 by air pressure of compressed air.The pressure switch 130 monitors the air pressure in the compressed air flow tube 120 and controls the operation of the air compressor 110 so that the air pressure becomes a predetermined pressure range.Diagram
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