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USE OF GENOMIC NW_006882077.1 IN CHO CELL FOR STABLY EXPRESSING A PROTEIN
USE OF GENOMIC NW_006882077.1 IN CHO CELL FOR STABLY EXPRESSING A PROTEIN
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机译:使用基因组NW_0068820777.1在CHO细胞中稳定表达蛋白质
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摘要
Use of genomic NW_006882077.1 in CHO cell for stably expressing a protein is disclosed. The certain site in CHO cell genome for stably expressing a protein is positioned at a base of No. 691045 in a CHO cell gene NW_006882077.1; a sequence of 5′ NNNNNNNNNNNNNNNNNNNNNGG3′ that can be identified by CRISPR/Cas9 technology and positioned in a base range of No. 690980-691090 around the certain site is a target sequence. Various of protein genes are introduced into a fixed site in CHO cell genome, and expressed stably in the present disclosure.
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