Method for producing potato plant suppressing browning using CRISPR/Cas9 system

摘要

The present invention relates to a method for producing a browning inhibition potato plant using a CRISPR/Cas9 system, and more particularly, to a ribonucleoprotein (RNP) complex containing sgRNA targeting a potato-derived StPPO2 ( Solanum tuberosum Polyphenol Oxidases 2) gene. The present invention relates to a method for producing a genome-modified potato plant in which browning is suppressed by transducing potato protoplasts. Since the method of the present invention does not insert a foreign gene and has only small mutations that cannot be distinguished from natural mutations, unlike Genetically Modified Organism (GMO) crops, which consume a lot of money and time to evaluate safety and environmental hazards. It is expected to save money and time.