Methods for in vitro continuous propagation of Babesia microti and microti-like species are disclosed. This method is B. Microtii as a source of complement and anti-bi in culture medium containing host red blood cells. incubating in the presence of a source of Microti IgM antibody. In one embodiment, anti-B. The source of Microti IgM antibodies is substantially free of IgG antibodies. In one embodiment, the source of complement is a cell or cell line that secretes complement.
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