Composition and method for evaluating attenuation and infectivity of Listeria strain

摘要

Methods and compositions for assessing the detoxification and / or infectivity of bacteria or Listeria strains, such as Listeria monocytogenes, are provided.In some embodiments, methods are provided for assessing the attenuation or infectivity of a test Listeria strain.This way isFor example:(a) it is an infectious THP-1 Cell that infects the test Listeria strainTHP-1 cells differentiate into macrophages before infection with Listeria strainsTo infect(b) dissolving THP-1 cellsDissolving dissolved matter on agar and(c) counting Listeria grown in THP-1 cells by growth on agar and.