There is provided a method for quantifying biovar in the industrial material utilizing polymerase chain reaction (PCR) and detection of amplified signals across multiple PCR thermal cycles.PCR targets DNA segments found in one or more biofouling materials often found in industrial materials.Samples collected from the material may be used directly or may be filtered, purified, dissolved or diluted, or may be used for the combination of such pretreatment.The substance is tested for use other than pharmaceuticals, medical applications other than medical treatment, papermaking facilities, and industrial applications such as leather processing facilities, or for use in the field or for preparation.A method of controlling or treating a system utilizing a substance or such substance, or a method of controlling or treating the surface intended to contact the material is provided.No selection
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