Microarray based multiple pathogen analysis and its use

摘要

A kit comprising a three-dimensional grid microarray system for detecting and analyzing DNA sequences and its components is provided herein.In the above system, the solid support comprises a plurality of bifunctional polymer linkers having optional fluorescence labels and each of the bifunctional polymer linkers having the top and bottom ends is bonded to the solid support at the bottom end, and the plurality of nucleic acid probes Binding to the top domain of a plurality of bifunctional polymer linkers.A method of fabricating a microarray system and method for detecting and identifying a pathogen in a plant sample, or by using a known copy number of synthetic DNA as an internal standard A method of determining the number of copies of the pathogen DNA in an unpurified nucleic acid sample is further provided.No selection