EXPRESSION VECTOR BASED ON CHIMPANZEE CHAD 63-TYPE ADENOVIRUS AND CONSTRUCTION METHOD THEREOF

摘要

A method of constructing an expression vector based on chimpanzee ChAd 63-type adenovirus. The method uses wild-type chimpanzee ChAd 63-type adenovirus to construct a vector capable of expressing exogenous genes. A chimpanzee ChAd 63-type adenovirus vector and a novel method for constructing an adenovirus vector from scratch are thus provided for the research and development of novel adenovirus vector-related products as well as for biomedical basic research thereof. The method is simple and precise, and allows for controllable quality and cost, shorter processing time, and lower sequence mutation risk. In addition, advance acquisition of wild-type strains is not required, improving the general applicability of the method.