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Sequencing and transcriptional analysis of high bandwidth polynucleotide Library

机译:高带宽多核苷酸文库的测序与转录分析

摘要

Provided herein are methods for target gene sequencing and single cell barcoding in conjunction with analysis of gene expression in single cells. In some embodiments, the target gene is an immune molecule, such as an antibody or TCR. In some embodiments, the methods can be used to carry out transcriptome sequencing, e.g., RNA sequencing, to capture transcriptome of single cells paired with full receptor immune receptor sequences such that information about the immune repertoire and transcriptome of a cell can be determined. Also provided are polynucleotide libraries for use in carrying out transcriptome analysis and immune molecule, e.g., antibody or TCR, sequencing.
机译:本文提供了用于靶基因测序和单细胞条形码的方法,结合分析单细胞中的基因表达。在一些实施方案中,靶基因是免疫分子,例如抗体或TCR。在一些实施方案中,该方法可用于进行转录组测序,例如RNA测序,以捕获与全受体免疫受体序列配对的单细胞转录组,使得可以确定关于细胞的免疫曲目和转录组的信息。还提供了用于进行转录组分析和免疫分子的多核苷酸文库,例如抗体或TCR,测序。

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