MITOCHONDRIAL G-QUADRUPLEX DNA-TARGETED FLUORESCENT PROBE, AND PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed in the present invention are a mitochondrial G-quadruplex DNA-targeted fluorescent probe, and a preparation method therefor and use thereof. The structure of the fluorescent probe is shown by formula (I), wherein R₁ is H, F, Cl, Br, or I; R₂ is -O(CH₂)_ntriphenyl phosphine, wherein n is any one integer of 2 to 10; R₃ is N-methylpiperazine, -NR₄R₅, or -NH(CH₂)_mR₆, wherein m is any one integer of 1 to 8; R₄ and R₅ each independently are hydrogen, C_1-8alkyl, or C_1-8haloalkyl; R₆ is amine, C_1-8alkylamino, or C_1-8alkoxyl group-substituted amino; and A^- is an N methylated anion, a halogen ion, a p-toluenesulfonic acid ion, or a trifluoromethanesulfonic acid ion. The fluorescent probe can specifically detect and recognize a mitochondrial G-quadruplex DNA structure, and is not interfered by other components; moreover, the fluorescent probe has good chemical stability, light stability, solubility, and biocompatibility, and has a wide application space in the biological function study of the mitochondrial G-quadruplex DNA.