NONINVASIVE QUANTITATION OF FULL VERSUS EMPTY CAPSIDS USING WATER PROTON NMR

摘要

A method of using the relaxation rate (R₁ and/or R₂) of solvent NMR signal to non-invasively assess whether viral capsids in a capsid preparation are full or empty, and the percentage of full capsids if the vial contains a mixture of full and empty capsids. The method can simply, rapidly, and non-invasively prove the safety and potency of the capsid preparation and thus whether the capsid preparation can be approved for clinical use, without requiring any sample preparation or reagent addition.