PRIMER, KIT AND METHOD FOR DETECTING TAIHE BLACK CHICKEN EGG

摘要

This invention discloses a primer, a kit and a method for testing Taihe black chicken egg. The described primer includes: as shown in SEQ ID No. 1, SEQ ID No. 2, SEQ ID No. 3 and SEQ ID No. 4. The described test method comprises the following steps: extract the genomic DNA of the egg to be tested, propagate the genomic DNA by two-step PCR, and perform genotyping according to the base at 68 bp of the gene sequence of the second-step PCR product. If the base at that site is G, the egg is a Taihe black chicken egg; if the base at that site is C, the egg is a non-Taihe black chicken egg. The variable site targeted by this invention is unique to Taihe black chicken egg.