BIOMEMBRANE FORCE PROBE SYSTEM-BASED SUPER-ALIGNMENT FORCE CLAMP EXPERIMENTAL METHOD

摘要

A biomembrane force probe system-based super-alignment force clamp experimental method. A probe end (1, 2, 3, 7) and a target end (4, 5) are involved in a force clamp experiment. The probe end (1, 2, 3, 7) comprises a probe pellet (3) and red blood cells (2). In the force clamp experiment of a biomembrane force probe, a reference pellet (7) is added to the probe end (1, 2, 3, 7), and by means of the strong interaction between protein molecules, the reference pellet (7) is bonded to the end part of a first micropipette (1) that suctions the red blood cells (2). Moreover, a relative distance (10) between the reference pellet (7) located at a side boundary (9) close to the red blood cells (2) and the probe pellet (3) located at a side boundary (8) close to the red blood cells (2) is tracked in real time, and the movement of the target end (4, 5) is controlled by means of a feedback algorithm to carry out the force clamp experiment. By means of adding the reference pellet (7) and developing a biomembrane force probe system-based 'double-boundary' force tracking mode, the accuracy of the force acting on the molecular bonds is significantly improved, accurate and stable acting forces may be applied to molecular bonds that have slow dissociation rates, and the recording time limit of the binding time of the molecular bonds is significantly prolonged for long-time recording.