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REDUCING ANTIBIOTIC RESISTANCE IN BACTERIA USING PRO-ACTIVE GENETICS
REDUCING ANTIBIOTIC RESISTANCE IN BACTERIA USING PRO-ACTIVE GENETICS
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机译:使用积极活性遗传学降低细菌中的抗生素抗性
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摘要
CRISPR-based gene-drive system for inhibiting antibiotic resistance of bacteria, including Escherichia coli that efficiently copies a gRNA cassette and adjacent cargo that are flanked with sequences homologous to the targeted gRNA/Cas9 cleavage site. This "pro-active" genetic system (Pro-AG) functionally inactivates an antibiotic resistance marker on a high copy number plasmid with greater efficiency than control CRISPR-based methods. Pro-AG can effectively edit large plasmids or single-copy genomic targets, or introduce functional genes, with numerous applications to biotechnology and biomedicine.
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