Risk assessment method for cerebral infarction

摘要

Problem to be solved: to provide a method in which the operator can identify the type of cerebral infarction in a short time.It is characterized by detecting the p.r4810k polymorphism in the rnf213 gene present in the genomic DNA of the subject.Risk assessment of cerebral infarction1. The analyteThe process of mixing the surfactant with the PCR buffer containing proteinase K;(2) the mixed solution obtained in stepPCR primer pairs to amplify base sequences corresponding to rnf213p.r4810k polymorphismAn oligonucleotide fluorescent labeling probe that binds to a mutated base sequence corresponding to the polymorphism and a set of oligonucleotide fluorescent labeling probes that bind to a wild-type nucleotide sequence corresponding to the mutated base sequenceThe fluorescent dyes that bind to the oligonucleotides blend with different oligonucleotide fluorescent labeled probe setsPCR processes;And3. Detecting the PCR product;Method.No selection