Antibody drug conjugate with high drug load

摘要

Problem to be solved: to provide a transglutaminase mediated antibody drug conjugate with a high drug to antibody ratio (DAR), a method of producing the antibody drug conjugate, and a method of using the antibody.Glutamine containing tagEndogenous glutamineAnd / or by manipulating or manipulated transglutaminaseEndogenous glutamine reacted with altered substrate specificity2) amine donor unitsIncludes a linker and an amine donor material containing an active portion of the enzymeDAR is at least aboutProvide an antibody - drug conjugate.Diagram