TIME-RESOLVED FLUORESCENT IMMUNOCHROMATOGRAPHIC TEST STRIP FOR DETECTING VANCOMYCIN AS WELL AS PREPARATION METHOD AND APPLICATION THEREOF

摘要

The present invention provides a time-resolved fluorescent immunochromatographic test strip for detecting vancomycin as well as a preparation method and application thereof, and belongs to the technical field of drug concentration detection of in vitro diagnostic reagents. The test strip comprises a bottom plate and a sample absorption pad, a fluorescent microsphere pad, a nitrocellulose membrane coated with a vancomycin-carrier protein conjugate, and an absorbent pad which are sequentially overlapped and pasted on the bottom plate. The fluorescent microsphere pad is sprayed with a fluorescent microsphere-labeled vancomycin monoclonal antibody, and the vancomycin monoclonal antibody is prepared by using a vancomycin-bovine serum albumin conjugate as an immunogen. The test strip of the present invention has no cross reaction with various vancomycin analogs, and has the advantages of high detection accuracy and strong specificity.