Preparation Methods for Fermentative Extract of Torreya Nucifera Seed from JEJU Having Anti-Inflammatory Activities

摘要

The present invention is a non-seed pulverized product manufacturing step of pulverizing dried non-seed to prepare a non-seed pulverized product; After the non-seed pulverized product manufacturing step is completed, the non-seed pulverized product, distilled water, bamboo salt and MRS medium are mixed in a weight ratio of 1: 2 to 4: 0.001 to 0.01: 0.005 to 0.012 in a temperature range of 115 to 125 ° C. A non-seed medium manufacturing step of sterilizing for 10 to 20 minutes to prepare a non-seed medium; After the non-seed medium preparation step is completed, 0.5 to 1.5 parts by weight of Lactobacillus plantarum seed cells are inoculated into the non-seed medium, based on 100 parts by weight of the non-seed medium, and then 1 in the temperature range of 35 to 40 ° C. Non-seed fermented powder manufacturing step of culturing for 3 days, sterilizing for 10 to 20 minutes in a temperature range of 115 to 125° C., and then freeze-drying to prepare non-seed fermented powder; And after the non-seed fermented powder manufacturing step is completed, based on 100 parts by weight of the non-seed fermented powder, it is mixed with 900 to 1,100 parts by weight of a solvent, stirred for 20 to 30 hours, filtered with a filter paper, and the filtered filtrate is concentrated under reduced pressure. to provide a method for producing a non-seed fermented extract comprising the step of preparing the non-seed fermented extract by drying the non-seed fermented extract. The method for producing a non-seed ferment extract according to the present invention provides a method for preparing a non-seed ferment extract, which is a natural product that has antioxidant and/or anti-inflammatory activity and has few side effects, and can be used in cosmetics, food, and pharmaceuticals.