Anti-inflammatory activity and qualitative analysis of different extracts of Maytenus obscura(A.Rich.) Cuf.by high performance thin layer chromatography method

摘要

cqvip:Objective:To perform aqueous elhanol soluble fraction(AESK) and dichloromethane extract of aerial parts of Maytenus obscura(A.Rich.) Cuf.using high performance thin layer chromatography(HPTLC) and to test anti-inflammatory activity of these extracts.Methods:HPTLC studies were carried out using CAM At;HPTLC system pquipped with Linomat IV applicator,TLC scanner 3.Reprostar 3.CAMAG ADC 2 and WIN CAT5-4 software were used.The anti-inflammatory activity was tested by injecting different groups of rats(6 each) with formalin in hind paw and measuring the edema volume before and 1h later formalin injection.Control group received saline i.p.The extracts treatment was injected i.p.in doses of 100 and 200 mg/kg 1h before formalin administration.Indomethaein(30 mg/kg) was used as standard.Results:The results of preliminary phytochemical studies confirmed the presence of protein,lipid,carbohydrate,phenol,flavonoid.saponin,triterpenoid,alkaloid and anthraquinonc in both extracts.Chromatography was performed on glass-backed silica gel 60 F254 HPTLC plates with the green solvents toluene:ethyacetate:glacial acetic acid(5:3:0.2,v/v/v) as mobile phase.HPTLC.finger printing of AESF revealed major eight peaks with R_f values in the range of 0.28 to 0.80 and the dichloromethane revealed major 11 peaks with R_f values in the range of 0.12 to 0.76.The purity of sample was confirmed by comparing the absorption spectra at start,middle and end position of the hand.Treatment of rats(i.p.) with AESF and dirhloromethane in doses of 100 and 200 mg/kg inhibited singnificantly(P<0.05.n=6) formalin-induced inflammation by 50%.55.9%,45.5%,and51.4%.respectively.Conclusions:HPTLC finger printing of AESF and dichloromethane of Maytenus obscura revealed eight major spots for alcoholic extracts and nine major spots for dichloromethane extracts.These HPTLC profiles may be of great usefulness in the quality control of herbal products containing these extracts.The anti-inflammatory activity of both extracts also revealed the medicinal importance of these extracts.The plant can be further explored for the isolation of phyloconstiluents having anti-inflammatory activity.