Reporter System for Assessing Cleavage Activity of CRISPR/Cas9

摘要

The present invention relates to a novel reporter system capable of evaluating the cleavage efficiency of gene scissors (CRISPR / Cas9), and more specifically, a first promoter - a gene scissors target sequence - Lac repressor - Poly (poly) A - Second promoter (promoter) - Lac operator (operator) - Reporter (reporter) gene - Poly (poly) A is operably linked in sequence Evaluation of cleavage efficiency of the gene scissors, characterized in that configured A reporter construct and a method for evaluating the cleavage efficiency of gene scissors using the same. According to the present invention, since the reporter systems for evaluating the efficiency of conventional gene editing depend on a frameshift in the protein coding region, it is difficult to measure the activity of the gene scissors when a 3n base insertion or deletion occurs. Thus, the present invention has the advantage of being able to evaluate the gene scissoring efficiency regardless of the frame shift. Therefore, using the reporter system of the present invention, sgRNA with high efficiency can be simply selected, and gene editing can be efficiently performed in cells and animal models using this.