METHOD FOR DETERMINING GENETIC MARKERS FOR ASSESSING POLYGENIC RISK OF BREAST CANCER (HORMONE-NEGATIVE AND HORMONE-POSITIVE SUBTYPES)

摘要

FIELD: medical biotechnology.;SUBSTANCE: invention relates to the field of medical biotechnology and concerns a method for determining genetic markers of polygenic risk of breast cancer (hereinafter – BC). A method for analyzing 38 genetic markers for assessing the polygenic risk of developing hormone-negative and hormone-positive subtypes of BC is proposed: 11q13.1 (rs3903072), 12q24 (rs12920U), 19q13.31 (rs3760982), 2q14.2 (rs4849887), 2q35 (rs16857609, rs13387042), 6q14.1 (rs17529111), 8p21.1 (rs9693444), 8q21.11 (rs6472903), 8q24.21 (rs11780156), BRCA2 (rs11571833), CASP8 (rs1045485), CCND1 (rs78540526), CDCA7 (rs1550623), CDKN2A/B (rs1011970), CHEK2 I157T (rs17879961), DNAJC1 (rs11814448), EBF1 (rs1432679), ESR1 (rs12662670, rs2046210), FGFR2 (rs2981579), FOXQ1 (rs11242675), FTO (rs17817449), HNF4G (rs2943559), LSP1 (rs3817198), MKL1 (rs6001930), NTN4 (rs17356907), PDE4D (rs1353747), PEX14 (rs616488), PTHLH (rs10771399), RAB3C (rs10472076), RAD51L1 (rs999737), SSBP4 (rs4808801), TCF7L2 (rs7904519), TERT (rs2736108, rs7726159), TOX3 (rs3803662), ZNF365 (rs10995190). ;EFFECT: invention allows simultaneous determination of genotypes at selected loci using multiplex PCR and subsequent hybridization with an oligonucleotide biological microchip (biochip).;2 cl, 2 dwg, 2 tbl, 3 ex