Methods for Introducing Mutations That Alter the Probability of Intranucleic Acid Base Pairing of a Conserved Structured Nucleotide and Related Compositions

摘要

The present invention relates generally to methods for introducing mutations that alter the probability of intranucleic acid base pairing of a conserved structured nucleotide in a nucleic acid. The present invention also provide methods for making mutant pathogenic organisms suitable as live attenuated vaccines, animal and human diagnostics, and for identifying suitable drug targets.