METHODS FOR INTRODUCING MUTATIONS THAT ALTER THE PROBABILITY OF INTRANUCLEIC ACID BASE PAIRING OF A CONSERVED STRUCTURED NUCLEOTIDE AND RELATED COMPOSITIONS

摘要

The present invention relates generally to methods for introducing mutations that alter the probability of intranucleic acid base pairing of a conserved structured nucleotide in a nucleic acid. The present invention also provide methods for making mutant pathogenic organisms suitable as live attenuated vaccines, animal and human diagnostics, and for identifying suitable drug targets.