DEVICE FOR IN VITRO PLANT CULTURE BY TEMPORARY IMMERSION IN A NUTRITIVE LIQUID

摘要

The invention relates to a device for in vitro culture (1) of a plant material (M) by temporary immersion in a nutritive liquid (L), this device (1) comprising: at least one culture container (2) each comprising: •a. a culture chamber (3), which has an internal volume, and which is intended to contain the plant material (M) to be cultured; •b. a nutritive liquid chamber (4) which is intended to contain the nutritive liquid (L); •c. transfer means (5) for the temporary transfer of at least one portion of the nutritive liquid (L) from said nutritive liquid chamber (4) to the culture chamber (3); •d. a removable lid (6) for closing said culture chamber (3) and also sealing means (7) inserted between this lid (6) and this culture chamber (3); •e. communication means (8), comprised by the removable lid (6), and which are intended to ensure communication between the internal volume of the culture chamber (3) and the exterior of the culture container (2). The culture device (1) further comprises both pressure-reduction means (9) for establishing, in the culture chamber (3), a pressure which is lower than the pressure in the nutritive liquid chamber (4), and connecting means (10) for connecting the pressure-reduction means (9) to said communication means (8) of said at least one culture container (2). The invention also relates to a method for in vitro culture (1) of plant material (M) by temporary immersion in a nutritive liquid.