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Optical in vitro detection method and apparatus for movement of biological sample and/or movement of biological sample components
Optical in vitro detection method and apparatus for movement of biological sample and/or movement of biological sample components
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机译:用于运动的光学体外检测方法和设备,用于生物样品和/或生物样品组分的运动
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摘要
The present invention relates to a method and apparatus for optically detecting the motion of a biological sample and/or a motion of a biological sample component in vitro. The method comprises the steps of: (a) providing a wide field illumination device for illuminating a sample configured to illuminate the entire biological sample (1) and a detector (3) for detecting light (9, 9a, 9b) from the biological sample; (b) illuminating the biological sample (1) with the wide field illumination device; And (c) detecting the movement of the biological sample 1 according to the output signal 6c of the detector 3. The detector 3 includes a detection area 3a subdivided into a plurality of detection areas 4a, and forms a derivative with respect to the time of the detection signals 4c of each of the detection areas 4a. And (S1), correcting the derivative by generating or squaring an absolute value (S2), summing or averaging the differential and corrected detection signals 4c of all detection zones 4a (S3), and outputting It can be provided as a signal 6c.
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