METHOD FOR OBTAINING INDIVIDUAL FRACTIONS OF MATRIX HYALURONATE OF VARIOUS MOLECULAR WEIGHTS OF HIGH FRACTIONAL AND CHEMICAL PURITY IN A SINGLE TECHNOLOGICAL CYCLE

摘要

FIELD: polysaccharide chemistry.;SUBSTANCE: method for producing hyaluronate includes washing the byproduct with running water, grinding it, extracting, filtering and combining the extracts. As the raw material, frozen or fresh animal byproducts are used, selected from chicken comb, cattle skin and eyes. After washing, the byproduct is denatured by dehydrating it with a saturated solution of sodium chloride, decontaminated with a compound of active oxygen, and placed in distilled water to swell. The cycle of dehydration, decontamination and swelling is repeated at least once, after which the dehydration and decontamination are repeated. The denatured material is crushed and homogenized, and three-fold extraction is carried out from the crushed denatured material with 0.85-0.9% sodium chloride solution at a temperature of 35-40°C with the separation of the meal by vacuum filtration on metal grids. Then, the extracts are combined, and fermentolysis is carried out when vacuumed with an enzyme complex including the papain, pepsin, trypsin enzymes and phosphodiesterase at a temperature of 30-35°C for 24-36 hours at a pH of 6.5-7.2. The fermentation mixture is heated to 70-80°C, quickly cooled to 5-10°C, and the lipid membrane is separated. Then, sequential ultrafiltration is performed with a final membrane with a pore size of no more than 0.2 mcm. The hyaluronate is precipitated with cetylpyridinium chloride. The precipitate of cetylpyridinium hyaluronate is dissolved in 0.9-1.2% sodium chloride solution to a concentration of no more than 0.15%, and the solution is filtered through a membrane with a pore size of no more than 0.2 mcm. Then, a solution of hyaluronate with a concentration of no more than 0.15% is prepared, followed by purification and separation of the hyaluronate into fractions by cascade filtration in a continuous cycle by reverse osmosis, and the finished product is obtained. ;EFFECT: invention makes it possible to obtain substances of matrix hyaluronate of various molecular weights of high fractional and chemical purity from frozen or fresh animal byproducts, excluding its contamination with microbiological toxins with a maximum yield of hyaluronate of a wide molecular spectrum and the production of such related products as chondroitin sulfate, meal in a single technological cycle.;10 cl, 8 tbl, 7 ex