LUCIFERASE-COMPLEMENTATION-BASED BIOSENSOR, PREPARATION METHOD THEREFOR AND USE THEREOF

摘要

Disclosed is a luciferase-complementation-based biosensor, comprising a luciferase amino terminal fragment and a luciferase carboxyl terminal fragment, wherein one of the amino terminal of the luciferase amino terminal fragment and the carboxyl terminal of the luciferase carboxyl terminal fragment is connected to a G protein, and the other terminal is connected to a first marker, and the luciferase amino terminal fragment and the luciferase carboxyl terminal fragment are two complementary fragments of the same luciferase; an analyte antigen connected to the second marker, wherein the second marker is complementary to the first marker; an analyte antibody; and a luciferase substrate. By using a quaternary complementary system of a complementary luciferase amino terminal fragment and carboxyl terminal fragment, a complementary first marker and second marker, a complementary analyte/analyte antigen and analyte antibody and a complementary G protein and analyte antibody, the rapid detection of the analyte can be realized, wherein the detection sensitivity is high, the specificity is strong, and the background noise is low, and same can be used for the quantitative analysis of the analyte in complex samples.