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Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes
Cytochrome P450 BM3 enzyme variants for preparation of cyclopropanes
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机译:细胞色素P450 BM3酶变体,用于制备环丙烷
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摘要
The present invention provides methods for catalyzing the conversion of an olefin to any compound containing one or more cyclopropane functional groups using heme enzymes. In certain aspects, the present invention provides a method for producing a cyclopropanation product comprising providing an olefinic substrate, a diazo reagent, and a heme enzyme; and admixing the components in a reaction for a time sufficient to produce a cyclopropanation product. In other aspects, the present invention provides heme enzymes including variants and fragments thereof that are capable of carrying out in vivo and in vitro olefin cyclopropanation reactions. Expression vectors and host cells expressing the heme enzymes are also provided by the present invention.
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